Abstract
The performance of two polymerase chain reaction (PCR) methods targeting chromosomal ail and plasmid-borne yadA gene sequences in the analysis of a variety of virulent and avirulent Yersinia enterocolitica strains was examined. All of the 45 virulent isolates tested were positive in the ail PCR method, whereas the yadA PCR method gave positive results with only 39 of these isolates, possibly due to virulence plasmid loss during subculturing of the six PCR-negative isolates. None of a panel of avirulent Y. enterocolitica strains, other Yersinia spp. and non- Yersinia bacteria tested gave positive results with either of the PCR methods, thus demonstrating their specificity. Both PCR methods provided excellent detectability in the assay of broth suspensions of cells, giving positive results with fewer than 5 c.f.u. per reaction using different serotypes. We conclude that the ail PCR method will provide a reliable tool for the analysis of virulent Y. enterocolitica.
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