Comparative Analysis of Vaginal Microbiota in Women with Breast Cancer in Kazakhstan.

Farida K Balmaganbetova,Ainur Amanzholkyzy,Aiman T Kaldybayeva,Azhar N Zhexenova,Roza E Nurgaliyeva

doi:10.31557/apjcp.2021.22.4.1313

Abstract

Object:The relevance of the article is that the breast cancer is a leading oncological disease in women in developed countries and has the highest mortality caused by malignant neoplasms in women. The purpose of the study is to evaluate vaginal microbiota in women with various breast cancer subtypes and compared groups. Methods:The study involved 278 women with breast cancer, of whom 174 were patients receiving combination therapy; the control group consisted of 104 patients who had had breast cancer 2-4 years ago. Results:It was found that despite a significant decrease in the total number of Lactobacillus spp., there were no statistically significant changes in the numbers of microorganisms in patients with different subtypes of breast cancer. According to the results of the comparative analysis, the representatives of obligate anaerobic flora Peptostreptococcus spp. prevailed in vaginal microbiota in luminal A and luminal B subtypes, and the representative of the facultative anaerobic organisms Staphylococcus spp. – in unfavourable outcomes in Her2/Neu+ and triple-negative subtypes. Conclusion:The observed features of the vaginal microbiota in women with different subtypes of breast cancer require further studies for preventive purposes.

Highlights

A steady increase in the rates of breast cancer in the world and the Republic of Kazakhstan requires the conduct and continuation of various studies, though many studies have been already dedicated to the problem of breast cancer
The scrapings from the posterolateral wall of the vagina using a urogenital probe were placed in special transport media for bioassays containing isotonic saline solution with preservatives for scrapings, which were subsequently delivered to the polymerase chain reaction (PCR) laboratory of the Scientific and Practical Centre (SPC) of the Marat Ospanov West Kazakhstan Medical University, where the method of PCR in real-time was used for qualitative and quantitative assessment of vaginal microflora
Representatives of mycoplasmas: Mycoplasma hominis 103.3 [102.4-104.7], Mycoplasma genitalium 103.4 [102.1-104.2], Ureaplasma 103.6 [102.6-104,7] – increased, which indicate the presence of mycoplasmosis, ureaplasmosis, nonspecific vaginitis, and urethritis

Summary

Introduction

A steady increase in the rates of breast cancer in the world and the Republic of Kazakhstan requires the conduct and continuation of various studies, though many studies have been already dedicated to the problem of breast cancer. In the Republic of Kazakhstan, up to 4,000 new cases of this oncological disease are registered annually, while the age of women is constantly decreasing, which affects the most productive, socially active, and reproductive age group of the female population of the city. The urgency of the problem is due to the increase in the rates of breast cancer worldwide, in the Commonwealth of Independent States (CIS) countries and the Republic of Kazakhstan. The urgency of this problem is since breast cancer is a leading cause of mortality in women among malignant neoplasms (Abitova et al, 2018; Morrison et al, 2018)

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Conclusion