COMPARATIVE ANALYSIS OF URINARY TRACT INFECTIONS AMONG UNIVERSITY STUDENTS, BOYS AND GIRLS

Abstract

Urinary tract infection (UTI) represents a serious health problem. The severity of urinary tract infection depends both on the virulence of the bacteria and the susceptibility of the host. The aim of this study was to determine the prevalence of urinary tract infection and demographic differentials among students, and also to treat effectively the infection, prevent urosepsis and reduce the likelihood of renal damage and provide symptomatic relief to patients. The objectives were to determine the proportion of students who have UTI, identify the common causative organism and to determine difference in UTI occurrence among students based on demographic variables. Ten students within the period of study were investigated. Personal data were collected using a structured questionnaire. Early morning midstream clean urine samples were collected from all participants who were willing to participate for analysis of UTI. Urine samples were cultured using standard loop technique on blood agar, cystein lactose electrolyte deficient medium agar and macconkey agar. Gram staining and biochemical test. After subculture the isolates were both Gram negative and positive, catalase test positive, oxidase test negative, citrate test negative, coagulase test positive, indole test negative. Total colony counts in the samples from the girls 68 105, 99 105, 21 103, 0 1 , 34 5. Then from the boys were 14 2 21 104, 18 103, 14 102, 0 101. Sensitivity test were carried out using Kirby-bauer method (disck diffusion method). The organisms were tested with antibiotics disc. . The tests were carried out on Mueller-Hinton agar, and incubated for 37degree celcius for 24hrs. All the bacteria were screened against Nitrofurantoin ceftriaxone, ciprofloxacin, gentamicin, ofloxacin, augmentin, pefloxacin, clarithromycin, chloramphenicol, ampicillin,. three ( 3 ) out of all the antibiotics bacteria that was isolated were sensitive to Nitrofurantoin, ampicillin, ciprofloxacin, While 5 were resistant to the other five (5) antibiotics , ceftriaxone, gentamicin, ofloxacin, augumentin, pefloxacin, clarithromycin, chloramphenicol. Nitrofuration, ciprofloxacinand Ampicilin showed the highest zone of inhibition in the urine sample analysis .by calculating the mean of inhibition zone diameters in replicate plates. Conclusion the general results show that Screening for UTI is important in every students. Based on the results Nitrofurantoin and ciprofloxacin, and ampicillin are the drug of the choice due to high resistant rate to the commonly used antibiotic we highly recommend it since is the most sensitive antibiotic and should be used.