Abstract
The trypsin inhibitor activity (TIA) in fresh leaves in Hedysarum alpinum L., H. flavescens Regel & Schmalh. and H. theinum Krasnob. in Central Siberian Botanical Garden Siberian Branch Russian Academy Science (CSBG SB RAS, in the foreststeppe of Western Siberia) was investigated in the vegetative phase of seasonal development. Trypsin inhibitors (PR-6 proteins) play the role of immunity factors, since under various influences on plants, the genes encoding the production of this group of substances are activated. They are synthesized in different plant organs, and are necessary for plant control with biotic and abiotic stresses. TIA in the species was characterized by low (< 20 mg/g dry weight) and medium (≥ 20 mg/g dry weight) values, as we identified. It was found that the TIA values vary at a high and very high degree when determining in fresh leaves, and in the study in dry leaf flour - to medium degree. The preservation of TIA values in the species in the dried leaves is shown. On the basis of this fact, the possibility of studying TIA in these species in dried leaves is justified.
Highlights
It is known, that various parts of all angiosperms contain substances that promote the protection of the plants from damage and phytophages
The purpose of the article is to show the results of a comparative study of trypsin inhibitor activity (TIA) in leaves in species of Hedysarum alpinum, H. flavescens, H. theinum in culture in the forest-steppe of Western Siberia
Values are expressed in milligrams TIA pure bovine trypsin inhibitor bound per 1 g of air-dried leaves flour
Summary
That various parts of all angiosperms contain substances that promote the protection of the plants from damage and phytophages. One of the groups of such substances are inhibitors of proteinases, and in particular, trypsin inhibitors (TI). For practical purposes in storage organs in many cultivated plants these substances are isolated and their properties are studied. These are immunity factors, since different influences on plants causes activation to occur on the genes encoding the production of this group of substances, performing protective functions. A multicomponent protection system: the creation of protective barriers, the activation of a hypersensitivity reaction, the synthesis of antimicrobial low-molecular peptides that exhibit a wide range of activity against fungi, bacteria and viruses, such as PR-6 proteins in particular, or trypsin inhibitors (TI), exist in the plants. Other studies have shown the efficacy of these molecules as an antiprotozoal drugs and for the treatment of diabetes and Alzheimer's disease [3,4]
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