Comparative Analysis of Total Phenolics, Flavonoid content and Antioxidant profile of date palm (Phoenix dactylifera L.) with different watering water from Oued Soufin Algeria

Abstract

The aim of this study is to investigate the antioxidant ability of the date fruits palm (Phoenix dactylifera L) of variety (Ali Ourached) which had been watered with different sources of water (Ground water and Tap water) from Oued Souf, Algeria. The methanolic extracts of Ali Ourached date fruit watering with Ground water (AG) and Tap water (AT) were extracted successively with chloroform, ethyl acetate and butanol. The extracts of Ali Ourached date fruit were found to be a rich source of phenols and flavonoids. The higher content of phenols was found in crude extract of fruits (AG) and crude extract of fruits (AT) with value equal to (124.2469±0.0000 and 93.4840±0.0264 mg GAE/100g DW respectively), the higher content of flavonoids was recorded in crude extract of fruits (AT) and butanol fraction (AT) with (4.3717±0.0016 and 2.6725±0.0003mg QE/100g DW respectively). To evaluate the antioxidant activity, we use two methods which were (AA), phosphomolybdenum method (PM) and 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH). For the antioxidant activity the AG extract was exhibited the highest reducing ability against the phosphomolybdenum cation with (303.1836±2.3229μmol of ascorbic acid/g of extract). The DPPH method revealed that ethyl acetate fraction (AT) having the highest antioxidant activity with IC50 equal to (0.1121±0.0043g/l) followed by ethyl acetate fraction (AG) (0.114±0.0039g/l). This study and our results it can be justification of presence of nature bioactive compounds which have antioxidant activity.