Abstract
Papaya (Carica papaya L.) is known to contain significant amount of phenolic compounds, alkaloids, glycosides and vitamins, which are responsible for its antioxidant activity. The objective of this study was to compare the variations in antioxidative and radical scavenging potential of aqueous leaf extracts of regenerated plant (in vitro) and green house grown plant (in vivo) of Carica papaya L. (var. Pusa Dwarf). In order to quantify the antioxidant activity, extracts were evaluated for 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl, ferric reducing antioxidant potential and superoxide dismutase activity. The total phenol content was determined by using Folin–Ciocalteu method. In all assays in vitro leaf extract showed highest radical scavenging activity. In vitro leaf samples were also found rich in phenolic content in comparison to callus and in vivo leaf samples.
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