Comparative Analysis of the Transcriptome of Latent Autoimmune Diabetes in Adult (LADA) Patients from Eastern China.

Yuqiao Ji,Xiaolong Chen,Dongmei Jiang,Hao Jin,Jian Liu,Hongmei Chen,Zhujun Yin,Tian Xia,Mingzhong Sun,Lei Li

doi:10.1155/2019/8616373

Abstract

Latent autoimmune diabetes in adults (LADA) is characterized as a slow-progressing form of autoimmune diabetes. LADA resembles some phenotypes of type 1 diabetes (T1D) and type 2 diabetes (T2D), frequently leading to misdiagnosis and inappropriate therapeutic strategies. Understanding its transcriptome profiles aids in revealing the detailed molecular mechanisms of LADA and its therapy. In the present study, we performed RNA-seq analysis of LADA patients from Eastern China and showed that LADA exhibited 277 differentially expressed genes (DEGs) with 199 upregulated and 78 downregulated. Gene ontology and KEGG pathway enrichment analysis revealed that these DEGs were mainly related to immune function and cell death and growth. Furthermore, a comparison of DEGs in LADA with those in T1D and T2D identified from the online databases showed that there are very few overlapped genes between LADA and T1D or T2D, confirming LADA to be a distinct type of diabetes from T1D or T2D. In summary, our comprehensive analysis may aid in the understanding and treatment of LADA patients in Eastern China.

Highlights

Diabetes, a metabolic disorder characterized by hyperglycemia, belongs to one of the top diseases causing disability in China and a huge health burden in China [1]
Hierarchical clustering heat map of the differentially expressed genes (DEGs) revealed that 4 latent autoimmune diabetes in adults (LADA) patients were clustered together and were distinctly separated from the clustering of 5 healthy controls (Figure 1)
We did a comparative analysis of the transcriptome profiles of LADA patients in Eastern China, followed by the ontology and KEGG pathway analyses showing these DEGs in LADA to be highly related to immune function and cell death and growth, respectively

Summary

Introduction

A metabolic disorder characterized by hyperglycemia, belongs to one of the top diseases causing disability in China and a huge health burden in China [1]. It is the seventh leading cause of death in the United States, which has been recently shown to be far underreported [2, 3]. Around 10% of them are diagnosed as latent autoimmune diabetes in adults (LADA) [6] These patients are usually over age 30 and had the presence of diabetes-associated autoantibodies (e.g., glutamic acid decarboxylase (GAD65 Ab)) [6]. LADA, resembling some of the phenotypes of T1D and T2D, is often defined as type 1.5 diabetes

Methods

Results

Conclusion