Comparative analysis of the complete chloroplast genomes from six Neotropical species of Myrteae (Myrtaceae).

Nureyev F Rodrigues,Natalia Balbinott,Frank Guzman,Igor Paim,Rogerio Margis

doi:10.1590/1678-4685-gmb-2019-0302

Nureyev F Rodrigues, Natalia Balbinott + Show 3 more

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https://doi.org/10.1590/1678-4685-gmb-2019-0302

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Abstract

Myrteae is the largest and most diverse tribe within Myrtaceae and represents the majority of its diversity in the Neotropics. Members of Myrteae hold ecological importance in tropical biomes for the provision of food sources for many animal species. Thus, due to its several roles, a growing interest has been addressed to this group. In this study, we report the sequencing and de novo assembly of the complete chloroplast (cp) genomes of six Myrteae species: Eugenia brasiliensis, E. pyriformis, E. nitida, Myrcianthes pungens, Plinia edulis and Psidium cattleianum. We characterized genome structure, gene content, and identified SSRs to detect variation within Neotropical Myrteae. The six newly sequenced plastomes exhibit a typical quadripartite structure, gene content and organization highly conserved among Myrtaceae species. Some differences in genome length, protein-coding genes and non-coding regions were found. Besides, IR boundaries present structural changes among species. Increased sequence diversity was observed in some intergenic regions, suggesting their suitability for investigating intraand interspecific genetic diversity in populational studies. These data also contribute to the improvement of taxa sampling in further phylogenetic investigations to understand Myrtaceae evolution.

Highlights

Myrtaceae encompasses over 6000 species of shrubs and trees, classified in 144 genera and subdivided into 17 tribes (Wilson et al, 2005; WCSP, 2019)
In the Neotropical region, most of Myrtaceae is represented by the tribe Myrteae, which comprises over 50 genera and 2500 species, representing half of the diversity of the family (Wilson et al, 2005; WCSP, 2019)
Leaves from Eugenia brasiliensis, Eugenia pyriformis, Eugenia nitida, Myrcianthes pungens, Plinia edulis and Psidium cattleianum trees were from a private in vivo collection in Gravataí, RS, Brazil (latitude (S): 29°51’52"; longitude (W): 50°53’53") and used to isolate chloroplasts by the modified high salt method, followed by cpDNA extraction with the CTAB method (Vieira et al, 2014)

Summary

Introduction

Myrtaceae encompasses over 6000 species of shrubs and trees, classified in 144 genera and subdivided into 17 tribes (Wilson et al, 2005; WCSP, 2019). The plastome scaffolds were orientated by MUMmer (Delcher et al, 2003) using Eugenia uniflora (NC_026450.1), Plinia trunciflora (NC_034801.1) or Psidium guajava (NC_033355.1) as reference genomes for species of the same or closer genus.

Results

Conclusion