Abstract
There are a number of different animals that belong to long- and short-lived species and show a various rate of ageing, providing an ideal model to investigate mechanisms of longevity. In this work, a metabolome profiling of blood plasma from fishes with various ageing rates—negligible (Pike Esox Lucius and Sterlet Acipenser ruthenus), gradual (Zander Sander lucioperca and Perch Perca fluviatilis) and rapid (Chum Salmon Oncorhynchus keta and Pink Salmon Oncorhynchus gorbuscha)—was assessed by means of direct infusion to quadrupole time-of-flight mass spectrometry. Of the 2056 distinct m/z features detected by a mass spectrometry metabolic profiling of blood plasma samples, fifteen metabolites in the classes of dipeptides, fatty acids, glycerolipids, phosphoethanolamines and phosphatidylcholines were significantly associated with ageing rate, independent of species differences. This is the first study of the metabolome of fishes with various ageing rate, and this untargeted approach highlighted the metabolic conditions that may serve to assess the ageing process.
Highlights
Ageing is a well-defined process for humans and many animals that is characterized by a progressive decline in physiological and reproductive capacity and increasing incidence rate and mortality over time
Principal component analysis has shown the existence of differences in metabolite plasma composition in the negligible (Esox Lucius and Acipenser ruthenus) (×), gradual (Sander lucioperca and Perca fluviatilis) (∆) and rapid (Oncorhynchus keta and Oncorhynchus gorbuscha) (#)
Theoretical isotope patterns for the metabolites were generated by the Isotope Pattern Calculator (Bruker Daltonics, Billerica, MA, USA). This is the first study of the metabolome of fishes with various ageing rate and the combination of the metabolite profiling DI-electrospray ionization (ESI)-QTOF mass spectrometry (MS) with statistical analyses have allowed for differentiating the blood plasma samples of fish species in accordance with their type of senescence
Summary
Ageing is a well-defined process for humans and many animals that is characterized by a progressive decline in physiological and reproductive capacity and increasing incidence rate and mortality over time. Rapid senescence meaning death after the first round of reproduction is observed in
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