Abstract
Isozyme systems of peroxidase (E.C. 1.11.1.7), esterase (3.1.1.2), acid phosphatase (E.C. 3.1.3.2) and total protein composition have been studied in soybean somatic embryos that were induced on immature zygotic embryos or were maintained as long-term (repetitive) embryogenic culture on MSB-medium with 10 μM 2,4-D. Protein and isozyme profiles were compared to those of zygotic embryos and seeds of soybean. Peroxidase isozyme spectra found in callus and in somatic embryos were much more diversified in contrast to low peroxidase activity and poor isoperoxidase spectrum in zygotic embryos. Isoesterase as well as acid phosphatase profiles of the somatic embryos are very similar to those of zygotic embryos, isoesterase spectrum of the callus differs qualitatively from the spectrum of the somatic embryos. Some typical soybean seed proteins (subunits of storage proteins, trypsin inhibitor) are synthesized in the somatic embryos.
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