Comparative analysis of skin prick test with immunoblotting of different manufacturers in the context of determining the sensitization to birch allergen in patients with respiratory allergic diseases

Abstract

The aim of the study was to evaluate the diagnostic parameters of various methods for determining birch allergen sensitization in patients with respiratory allergic diseases – allergic rhinitis and bronchial asthma. Materials and methods: During the study, 88 patients with allergic rhinitis and/or atopic asthma were examined with three different methods of specific allergic diagnosis (in vivo and in vitro) according to the recommendations of the ethics committee of the Pirogov Vinnitsa National Medical University, all of which were out of acute. Inclusion criteria were a diagnosis of allergic rhinitis (both intermittent and persistent) and/or atopic asthma. The pre-test was conducted according to the classic test method according to the normative documents with commercial allergen extracts. Western blots for the determination of IgE levels were performed using RIDA AllergyScreen test systems (R-Biopharm AG, Darmstadt, Germany) and Euroline (Euroimmun). Results and discussion: Systematic error of measurement results of immunoblot of different manufacturers is equal to 2.27 ku/l, which indicates the presence of a systematic difference. The distribution graph corresponds to the type of absolute systematic error graphs. The standard deviation of the difference was 11.28, which is significantly compared to the values themselves. Thus, the results of the two systems for the determination of specific IgE for birch allergen by the Rida AllergyScreen and Euroline methods are not well matched due to systematic divergence of indicators. There is a good agreement between the results of skin testing with birch allergens and the detection of specific IgE by the Rida AllergyScreen test, and between the results of skin testing with birch allergen and the detection of specific IgE by the Euroline method, there is a moderate agreement between the results.