Abstract
Five antioxidant and two oxidative stress assays were applied to serum samples of 43 healthy males. The antioxidant tests showed different inter-assay correlations. A very good correlation of 0.807 was observed between the ferric reducing ability of plasma (FRAP) and total antioxidant status (TAS) assay and also a fair correlation of 0.501 between the biological antioxidant potential (BAP) and TAS assay. There was no statistically significant correlation between the BAP and FRAP assay. The anti-oxidant assays have a high correlation with uric acid, especially the TAS (0.922) and FRAP assay (0.869). The BAP assay has a much lower and no statistically significant correlation with uric acid (0.302), which makes BAP more suitable for the antioxidant status. The total thiol assay showed no statistically significant correlation with uric acid (0.114). The total thiol assay, which is based on a completely different principle, showed a good and statistically significant correlation with the BAP assay (0.510) and also to the TAS assay, but to a lower and not significant extent (0.279) and not with the FRAP assay (−0.008). The oxy-adsorbent test (OXY) assay has no correlation with any of the other assays tested. The oxidative stress assays, reactive oxygen metabolites (ROM) and total oxidant status (TOS), based on a different principle, do not show a statistically significant correlation with the serum samples in this study. Both assays showed a negative, but not significant, correlation with the antioxidant assays. In conclusion, the ROM, TOS, BAP and TTP assays are based on different principles and will have an additional value when a combination of these assays will be applied in large-scale population studies.
Highlights
The antioxidant assays used in this study were the ferric reducing ability of plasma (FRAP), biological antioxidant potential (BAP), total antioxidant status (TAS), total thiols in proteins (TTP) and oxy-adsorbent test (OXY) assays
Three closely related methods were used for determination of total antioxidants in serum samples from patients included in our study: the FRAP assay, the BAP method and the TAS method
For FRAP, a good correlation with uric acid was found [27]. All these results suggest that designed to measure the same entity, that being the level of total antioxidants in the sample, the three methods could sometimes lead to different conclusions for the same set of samples
Summary
Oxidative stress, which is defined as an imbalance between the pro-oxidant reactive species and antioxidant molecules, both endogenous and exogenous, has been associated with many non-communicable diseases, such as obesity, insulin resistance [1] and diabetes [2], atherosclerosis [3,4], autoimmune diseases [5,6], neurodegenerative diseases [7,8], chronic renal disease [9,10], different malignancies [11,12], as well as in aging [13] as a physiological process. As a result of the wide interest in the oxidative stress and antioxidants, numerous commercial test kits for oxidative stress assessment became available. These tests are dedicated primarily for use in clinical studies, their use in experiments with animals or cell cultures, even in foods and beverages, is not excluded. Having the possibility to choose from many methods and manufacturers, it is a real challenge to choose the right combination of methods and assays for specific health research. Time-consuming, there are still possibilities to prepare reagents in the laboratory, according to already published methods and protocols
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