Abstract
BackgroundThe collection of exhaled breath condensate (EBC) is a suitable and non-invasive method for evaluation of airway inflammation. Several studies indicate that the composition of the condensate and the recovery of biomarkers are affected by physical characteristics of the condensing device and collecting circumstances. Additionally, there is an apparent influence of the condensing temperature, and often the level of detection of the assay is a limiting factor. The ECoScreen2 device is a new, partly single-use disposable system designed for studying different lung compartments.MethodsEBC samples were collected from 16 healthy non-smokers by using the two commercially available devices ECoScreen2 and ECoScreen at a controlled temperature of -20°C. EBC volume, pH, NOx, LTB4, PGE2, 8-isoprostane and cys-LTs were determined.ResultsEBC collected with ECoScreen2 was less acidic compared to ECoScreen. ECoScreen2 was superior concerning condensate volume and detection of biomarkers, as more samples were above the detection limit (LTB4 and PGE2) or showed higher concentrations (8-isoprostane). However, NOx was detected only in EBC sampled by ECoScreen.ConclusionECoScreen2 in combination with mediator specific enzyme immunoassays may be suitable for measurement of different biomarkers. Using this equipment, patterns of markers can be assessed that are likely to reflect the complex pathophysiological processes in inflammatory respiratory disease.
Highlights
The collection of exhaled breath condensate (EBC) is a suitable and non-invasive method for evaluation of airway inflammation
Regarding leukotriene B4 (LTB4), we noticed that in EBC obtained with ECoScreen, all samples turned out to be detectable but under the limit of quantification (LOQ)
Concentrations of LTB4 in EBC samples collected with ECoScreen2 were in the linear part of the calibration curve of the competitive immunoassay
Summary
The collection of exhaled breath condensate (EBC) is a suitable and non-invasive method for evaluation of airway inflammation. Collection of EBC is a non-invasive tool for assessing pathophysiologic processes in airway diseases [2]. Since EBC contains no cellular components the evaluation and quantification of airway or lung pathology is based on detection of biomarkers [3]. When studying different biomarkers in one EBC sample, the methodical setting often is based on a compromise and should be appropriately evaluated [6]. This is true for the analytical assays concerning sensitivity and specificity versus availability, cost, and technician time required [7]
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