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Abstract
RNAi is an efficient surveillance machinery that plays a robust defensive role in shielding plant and animal hosts against viral infections. In counter-defense viruses encode suppressor proteins that have the ability to restrict the RNAi machinery to ensure successful systemic invasion. The B2 protein of insect Flock House Virus (FHV-B2) and AC2 protein of Mungbean Yellow Mosaic India Virus (MYMIV-AC2) are two well-characterized suppressors of RNAi, capable of reversing reporter gene silencing. In this study, we compared the strength of the two suppressors by assaying for the degree of RNAi reversion and the duration of sustaining the reversal in planta. The suppression activity was observed by assaying for GFP fluorescence at 3 dpi, 7 dpi and 14 dpi. The phenotypic observations were corroborated with small RNA Northern Blotting and semi-quantitative RT-PCR. The results indicate that suppressor strength of FHVB2 is comparable to MYMIV-AC2, although they are encoded by virus infecting host from two different eukaryotic kingdoms. This study will provide new insights to dissect the conservation in the RNAi pathways during the host-virus interactions.
Highlights
RNA silencing is a conserved sequence specific method of gene regulation and one of the potent antiviral de-How to cite this paper: Das, S.S. and Sanan-Mishra, N. (2014) Comparative Analysis of RNAi Suppression Activity of Proteins from Two Disparate Viruses
It was observed that the plant mutants defective in one or several of the RNA silencing pathways were more susceptible to virus infection [6]-[9]
Since most of the earlier work on the RNA silencing suppressors (RSS) involved model systems that differ in terms of host, transgene used or the method of delivery, it was difficult to correlate the degree of silencing ability and viral pathogenicity [36]
Summary
How to cite this paper: Das, S.S. and Sanan-Mishra, N. (2014) Comparative Analysis of RNAi Suppression Activity of Proteins from Two Disparate Viruses. It was observed that certain pairs of co-infecting viruses caused synergistic viral diseases This led to the identification of proteins, encoded by many viruses, called the RNA silencing suppressors (RSS) that have the ability to counteract antiviral silencing and play an important role in virulence [10] [11]. Since most of the earlier work on the RSS involved model systems that differ in terms of host, transgene used or the method of delivery, it was difficult to correlate the degree of silencing ability and viral pathogenicity [36] To address this issue we have tried to define parameters for comparing the suppressor strength of two well-known RSS, viz FHVB2 and MYMIV-AC2 protein by utilizing an in-planta reversal of GFP silencing assay
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