Comparative analysis of remediation efficiency and ultrastructural translocalization of polycyclic aromatic hydrocarbons in Medicago sativa, Helianthus annuus, and Tagetes erecta

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are semi-volatile anthropogenic contaminants that can damage soil fertility and threaten the environment due to their hazardous effects on various ecological parameters. The experimental objective was divided into two parts because PAHs are always present in mixtures. The toxicity of anthracene, phenanthrene, pyrene, and fluoranthene was examined and investigated the potential of three phytoremediator plants species viz Tagetes erecta, Helianthus annuus, and Medicago sativa for remediation and translocation of individual PAH. PAHs were shown to have inhibitory or stimulating effects on growth, antioxidant properties, and impact on the structure of plant cells. The result showed that M. sativa significantly enhances the removal rate of PAHs in the soil. The dissipation rate reached 96.2% in M. sativa planted soil, followed by H. annuus and T. erecta. Among the plant species, M. sativa exhibited the highest root and shoot concentrations (314.37 and 169.55 mg kg−1), while the lowest concentration was 187.56 and 76.60 mg kg−1 in T. erecta. SEM-EDX and fluorescence micrographs confirmed that pyrene altered plant tissue’s ultrastructure and cell viability and was found to be the most toxic and resistant. M. sativa was proven to be the most effective plant for the mitigation of PAHs.