Abstract
The utility of horizontal attenuated total reflection HATR FT-IR technique for preliminary plant leaf cuticular waxes qualitative analysis was studied. Detection and identification of small amounts of surface compounds were achieved. The main components of analysed samples were long-chain aliphatic compounds, mainly hydrocarbons. Weak absorption in the range of 1717–1738cm−1 indicated the presence of some compounds containing carbonyl groups. In the case of direct HATR analysis of potato leaf surface, an additional broad band of water was observed. The results obtained by infrared methods are in good agreement with HPLC analyses. HATR FT-IR is convenient and a rapid method of preliminary evaluation of surface plant material composition, especially for esters, ketones and aldehydes.
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