Abstract
Study of the distribution of the oxygen mass transfer coefficient, k (l) a, for a stirred bioreactor and simulated (pseudoplastic solutions of carboxymethylcellulose sodium salt) bacterial (P. shermanii), yeast (S. cerevisiae), and fungal (P. chrysogenum free mycelia) broths indicated significant variation of transfer rate with bioreactor height. The magnitude of the influence of the considered factors differed from one region to another. As a consequence of cell adsorption to bubble surface, the results indicated the impossibility of achieving a uniform oxygen transfer rate throughout the whole bulk of the microbial broth, even when respecting the conditions for uniform mixing. Owing to the different affinity of biomass for bubble surface, the positive influence of power input on k (l) a is more important for fungal broths, while increasing aeration is favorable only for simulated, bacterial and yeast broths. The influence of the considered factors on k (l) a were included in mathematical correlations established based on experimental data. For all considered positions, the proposed equations for real broths have the general expression [Formula in text] exhibiting good agreement with experimental results (with maximum deviations of ± 10.7% for simulated broths, ± 8.4% for P. shermanii, ± 9.3% for S. cerevisiae, and ± 6.6% for P. chrysogenum).
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