Comparative Analysis of MRSA Isolation, Biofilm Formation, and Molecular Characteristics: Hospital-Acquired Versus Community-Acquired Strains

Abstract

The current study was to look into the molecular characterisation and biofilm development of MRSA in patients who visited the Khyber Teaching Hospital, Hayatabad Medical Complex, and Lady Reading Hospital in Peshawar. Antibiotic susceptibility tests identify Methicillin-resistant Staphylococcus aureus in confirmed isolates of S. aureus. PCR was used to identify a gene, which proved that the MRSA isolates were real. HA- and CA-MRSA were distinguished by SCC mec typing. The microtiter plate assay was employed to look for signs of biofilm formation. 133 (82.60%) of the 161 samples that were positive for S. aureus. Of them, 32 (19.87%) had MSSA isolates, 28 (17.39%) were culture-negative, and 101 (62.73%) were determined to carry MRSA. To confirm all positive isolates one more time, Mec A gene analysis was performed. Twenty-one (20.79%) of the eighty (79.20%) confirmed isolates were classified as undistinguishable MRSA isolates, twenty-six (32.5%) as HA-MRSA isolates, and fifty-five (67.5%) as CA-MRSA isolates.