Abstract
Women with polycystic ovarian syndrome (PCOS) often have a history of infertility and poor pregnancy outcome. The character of the lower genital tract (LGT) microbiome of these patients is still unknown. We collected both vaginal and cervical canal swabs from 47 PCOS patients (diagnosed by the Rotterdam Criteria) and 50 healthy reproductive-aged controls in this study. Variable regions 3–4 (V3–4) were sequenced and analyzed. Operational taxonomic unit (OTU) abundance was noted for all samples. Taxa that discriminated between PCOS and healthy women was calculated by linear discriminant analysis effect size (LEFSe). Results from 97 paired vaginal and cervical canal samples collected from 97 women [mean age 30 (±4 years)] were available for analysis. Using the Rotterdam Criteria, 47 women were diagnosed with PCOS (PCOS, n = 47; control, n = 50). There was no significant difference between cervical canal microbiome and vaginal microbiome from the same individual, however, Lactobacillus spp. was less abundant in both vaginal and cervical canal microbiome of PCOS patients. Several non-Lactobacillus taxa including Gardnerella_vaginalis_00703mash, Prevotella_9_other, and Mycoplasma hominis, were more abundant in the LGT microbiota of PCOS patients. There is a difference between the microorganism in the LGT of patients with PCOS and healthy reproductive-aged women.
Highlights
Polycystic ovarian syndrome (PCOS) is one of the most common endocrine syndromes of reproductive-aged women, which displays diverse symptoms including irregular menstruation, sterility, recurrent abortion, and metabolic disorder, affecting about 10 to 15% of women worldwide and costing millions of dollars per year
Operational taxonomic unit (OTU) that discriminated between polycystic ovarian syndrome (PCOS) patients and healthy controls in both sites were analyzed by LEfSe
Vaginal and cervical canal samples were obtained from 97 women (47 PCOS patients, 50 controls) between December 2018 to June 2019
Summary
Polycystic ovarian syndrome (PCOS) is one of the most common endocrine syndromes of reproductive-aged women, which displays diverse symptoms including irregular menstruation (due to oligo-ovulation or anovulation), sterility, recurrent abortion, and metabolic disorder, affecting about 10 to 15% of women worldwide and costing millions of dollars per year. LGT Microbiome of PCOS Patients microorganism in the vaginal, cervical, as well as endometrial areas of reproductive-aged women, the diversity of microbiota in women’s genital tract have been reported to be linked with several adverse reproductive issues such as infertility, recurrent abortion, poor outcome of IVF, and preterm birth (Sirota et al, 2014; Torcia, 2019; Tsonis et al, 2020). Discovering the situation of PCOS women’s LGT microbiome may help us obtain a more comprehensive understanding of this syndrome, and by the angle of lower genital tract microenvironment, may provide new insights into the mechanism and treatment on the reproductive issues of PCOS patients
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