Abstract
Comparative analysis of human DNA extraction methods and mitochondrial DNA HV1 and HV2 haplogroup determination
Highlights
Genome is the most studied model system in molecular biology, genetics and forensic research
As GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific) gave the highest and constant DNA yield in all samples, we chose to use DNA obtained with purification kit for sequencing
Compared with protocol 2 and protocol 3, this method gave the best mean purity value of DNA that can be a result of using ammonium acetate to eliminate proteins
Summary
Genome is the most studied model system in molecular biology, genetics and forensic research. Every field of study and further testing requires the use of many different DNA extraction methods which vary in processing time, yield, purity of isolated DNA and cost. DNA yield and purity are values that define the efficiency of DNA extraction method (BOESENBERGSMITH et al, 2012). DNA is usually stored in a chamber at -80°C or in a liquid nitrogen container at 196°C for permanent cryopreservation (PASKAL et al, 2018). As it is stored at a very low temperature, the quality and integrity of extracted DNA are crucial for DNA biobanking (COPPOLA et al, 2019). Biobanks open many opportunities in biomedical research (large collections and databases, comparative analyses, conservation biology, preserving biodiversity and population genetic research, repeated testing over the years), as well as in biological anthropology (questions related to human evolution, population genetics, genealogy, the origin of modern humans) and molecular systematics (defining phylogenetic trees, haplogroups and haplotypes) (BRANKOVIĆ et al, 2014; DROEGE et al, 2014)
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