Abstract
Genetic relationships of 16 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) and its two wild relatives (C. albicans and C. lineatus) from different parts of the India were analysed using 22 random amplified polymorphic DNAs (RAPDs) primers and 10 inter simple sequence repeats (ISSRs) primers. Twenty two RAPD primers yielded 151 polymorphic markers (71.2%) with an average of 6.8 polymorphic band/primer. Cluster analysis based on these 151 RAPD markers revealed relatively low level (0.434 - 0.714) of genetic diversity among cultivars and high level of diversity between cultivars and wild relatives. Ten ISSR primers produced 100 bands across 16 cultivars and its wild relatives out of which 93 (93%) were polymorphic with an average of 9.3 polymorphic band/primer. Cluster analysis based on these 93 ISSR markers also revealed relatively higher level (0.328 - 0.827) of genetic diversity among cultivars as compared to RAPD markers. The polymorphic markers obtained by both RAPD and ISSR primers were pooled and the genetic diversity analysis based on these 244 markers was analysed. Jaccard’s similarity coefficient obtained by pooled data revealed very narrow range (0.477 - 0.720) among cultivated and high range between cultivated and wild species C. albicans (0.240 - 0.331) and C. lineatus (0.163 - 0.193). In the UPGMA based dendrogram the 16 cultivars were grouped into three distinct clusters. Cluster I contained two genotypes, cluster II had six and cluster III had eight genotypes. Principal components analysis (PCA) also resulted in similar pattern as that of UPGMA based analysis. The first three PCs contributed 56.26%, 5.71% and 4.97% of variation, respectively, with cumulative variation of the first three PCs was 66.96%. Both the markers and the combined data revealed similar pattern with narrow diversity among cultivars and higher diversity between cultivars and wild one, but the genetic diversity range obtained by ISSR markers was relatively higher as compared to RAPD and pooled data. Furthermore, both the markers also correlated the clustering of stress resistant genotypes together. Cultivar Pusa-2002 possessed more diversity with other genotypes in ISSR dendrogram.
Highlights
Pigeonpea [Cajanus cajan (L.) Millsp.] is an important crop in semi-arid tropical and subtropical farming systems, providing high quality vegetable protein (20% - 22%), animal feed, and firewood
Polymorphism Detected by random amplified polymorphic DNAs (RAPDs) Markers In RAPD analysis, our earlier results [26] based on 30 primers tested were included in the analysis
The present work can be summarized that all the genotypes could be distinguished by RAPD and inter simple sequence repeats (ISSRs) markers, respectively
Summary
Pigeonpea [Cajanus cajan (L.) Millsp.] is an important crop in semi-arid tropical and subtropical farming systems, providing high quality vegetable protein (20% - 22%), animal feed, and firewood. Woody, perennial legume with centre of diversity in India [1] It is only cultivated food crop of the cajaninae sub-tribe and popularly known as red gram (Arhar or Tur). It has diploid genome (2n = 22) and estimated at about 0.853 pg [2]. The nitrogen-fixing ability of pigeonpea makes it an important component in sustainable cropping systems, and farmers recognize and appreciate the ability of pigeonpea to “replenish” the soil when planted after a cereal crop [3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
