Comparative Analysis of Functional Gene Diversity of Acid Mine Drainage and its Sediment by Geochip Technology

Abstract

The diversity of microbial community is well studied in past decades, however, the functional gene diversity in AMD and sediment are still unclear. In this study, four samples, which included two mine drainages and two sediments were taken from two typical copper mines in the southeast of China. Community DNA from the AMD and corresponding sediments were were extracted, purified, amplified, labeled and hybridized with GeoChip 2.0. The results showed that total 28, 126, 1131, 1875 functional genes were detected in DX_110, YP_NK, DX_110N, YP_NKN, respectively, which including carbon and nitrogen fixation, carbon degradation, methane metabolism, ammonification, nitrification, denitrification, nitrogen reduction, sulfur reduction and metal resistance genes. Sediment nearby the mine drainage may play an important role in microbial geochemical processes, since more functional genes and higher diversity were detected in sediment than in AMD.