Comparative Analysis of Four Analytical Methods for Measuring Microalgae and Cyanobacteria Biomass Growth in Controlled Environment Minkery Wastewater

Abstract

In the process of microalgae and cyanobacteria cultivation, the biomass measurements remain a challenging practice. Four analytical methods commonly used are dry weight, optical density, cell count, and chlorophyll a content. The purpose of this study was to comprehensively compare the practical utility and analytical reliability of these four most recognized analytical methods in controlled environment minkery wastewater. Based on the findings, dry weight measurement has been proven to be the most precise method, optical density measure was the most time-effective and most accurate indirect method, and cell count was the most cost-effective method. All three methods demonstrated excellent correlations with each other. The chlorophyll a measurement was not as practical as the other three methods. This study suggests dry weight measurements at the beginning and end of the growth period partnered with frequent optical density measurements and cell counts for monitoring microalgae and cyanobacteria biomass in minkery wastewater.