Abstract
Rhododendron sichotense Pojark. and Rhododendron adamsii Rheder have been actively used in ethnomedicine in Mongolia, China and Buryatia (Russia) for centuries, as an antioxidant, immunomodulating, anti-inflammatory, vitality-restoring agent. These plants contain various phenolic compounds and fatty acids with valuable biological activity. Among green and selective extraction methods, supercritical carbon dioxide (SC-CO2) extraction has been shown to be the method of choice for the recovery of these naturally occurring compounds. Operative parameters and working conditions have been optimized by experimenting with different pressures (300–400 bar), temperatures (50–60 °C) and CO2 flow rates (50 mL/min) with 1% ethanol as co-solvent. The extraction time varied from 60 to 70 min. A HPLC-UV-VIS-ESI-MS/MS technique was applied to detect target analytes. A total of 48 different biologically active components have been identified in the Rh. adamsii SC-CO2 extracts. A total of 31 different biologically active components have been identified in the Rh. sichotense SC-CO2 extracts.
Highlights
A total of 19 species of rhododendrons are growing in the territory of Russia, the main part of which (13 species) are found only in the flora of the Russian Far East and Eastern Siberia [1]
This study considers the possibility and effectiveness of supercritical carbon dioxide (SC-CO2 )
The results show that L. palustre essential oil enhanced a significant inhibition of oedema (50–73%) for HD oil and (52–80%) for Supercritical fluid extraction (SFE) oil
Summary
A total of 19 species of rhododendrons are growing in the territory of Russia, the main part of which (13 species) are found only in the flora of the Russian Far East and Eastern Siberia [1]. Russian researchers classify the genus Rhododendron somewhat differently than foreign ones [2]. There is no unified classification scheme for a taxon, since the genus is very large—more than 800 species, as well as the presence of a large number of convergent characters among its representatives, complicating the construction of a natural classification [3]. The genus system, developed and adopted by Russian scientists, divides the genus Rhododendron into subgenera and series. Rhododendron adamsii Rehder and Rhododendron parvifolium Adams are assigned to the subgenus Osmothamnus Maximowicz
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