Comparative Analysis of Deep Learning and Machine Learning-based Models for Simultaneous Prediction of Minerals in Perilla (Perilla frutescens L.) Seeds Using Near-Infrared Reflectance Spectroscopy

Abstract

Perilla seeds contain a rich array of essential minerals, thus having the potential to address multiple micronutrient deficiencies at a time. However, traditional methods of mineral estimation are complex, time-consuming, expensive, and require technical expertise. This study includes the development of Near-Infrared Reflectance Spectroscopy (NIRS)-based prediction models for predicting five important minerals (Calcium, Copper, Magnesium, Manganese, and Phosphorus) using machine learning and deep learning techniques. Four models, including 1D Convolutional Neural Networks (1D CNNs), Artificial Neural Networks (ANNs), Random Forests (RFs), and Support Vector Regression (SVR), were developed and evaluated. The developed 1D CNN model outperformed other considered models in predicting calcium, magnesium, and phosphorus content with RPD (Residual Prediction Deviation) values of 1.75, 1.83, and 2.96, respectively. Whereas SVR performed best in predicting copper and manganese with an RPD of 1.82 and 2.2, respectively. The 1D CNN model demonstrated R2 (Coefficient of determination) values above 0.65 for all minerals, with a maximum of 0.88 for phosphorus. In addition, the developed models performed superior as compared to the Partial Least Square Regression method (R2= 0.32). The developed models provide efficient tools for rapidly screening perilla germplasm available in global repositories, thus aiding in the selection of mineral-rich genotypes to mitigate micronutrient deficiencies.