Abstract
AbstractWe performed a comparative study of the detection of human cellular prions (PrPC) with a quartz crystal microbalance (QCM) immunosensor using various antibodies against PrPC. The sensing layer was formed by immobilized antibodies on a surface of polyamidoamine dendrimers (PAMAM) of fourth generation (G4) with attached protein A. The sensitivity of detection depended on the kind of antibody used and the best detection limit was 0.8 nM. PrPC detection in 10 times diluted blood plasma revealed more complicated behavior of motional resistance, which is evidence on viscosity contribution. The sensor recovery was 82.2 %, suggesting certain matrix effects on prion detection.
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