Comparative analysis of blood and saliva expression profiles in chronic and refractory periodontitis patients.

Bin Zhang,Ting Lin,Hong He

doi:10.1186/s12903-015-0150-3

Bin Zhang, Ting Lin + Show 1 more

Open Access

https://doi.org/10.1186/s12903-015-0150-3

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Abstract

BackgroundThis study aimed to identify characteristic representative genes through a comparative analysis of gene expression profiles in the blood and saliva of chronic periodontitis (CP) and refractory periodontitis (RP) patients to provide new treatment strategies that may be helpful in the treatment of different forms of periodontitis.MethodsGSE43525 was downloaded from Gene Expression Omnibus. In the dataset, thirteen samples were from blood including 4 controls, 4 CP and 5 RP samples, and ten samples were from saliva including 3 controls, 4 CP and 3 RP samples. After comparing the CP and RP samples, differentially expressed genes (DEGs) between these two types of periodontitis in the blood and saliva samples were identified by an LIMMA package. Then, functional and pathway enrichment analyses were performed by DAVID and KOBAS, respectively. The significantly associated miRNAs in CP and RP were searched by WebGestalt.ResultsIn total, 213 DEGs in CP and 45 DEGs in RP were identified. Functional enrichment showed that the DEGs of CP were mainly enriched in ribosome and regulation of apoptosis-related pathways in blood as well as saliva, while the DEGs of RP were significantly enriched in immune responses and response to organic substance-related pathways. Several miRNAs, such as miR-381 and miR-494, were identified as being closely associated with CP. In addition, CD24, EST1, MTSS1, ING3, CCND2 and SYNE2 might be potential targets for diagnosis and treatment of CP.ConclusionThe identified DEGs and miRNAs might be potential targets for the treatment of chronic and refractory periodontitis.

Highlights

This study aimed to identify characteristic representative genes through a comparative analysis of gene expression profiles in the blood and saliva of chronic periodontitis (CP) and refractory periodontitis (RP) patients to provide new treatment strategies that may be helpful in the treatment of different forms of periodontitis
Functional enrichment analysis of differentially expressed genes (DEGs) To study the functions of DEGs in CP and RP in blood and saliva, the functional enrichment analysis of all these DEGs were performed by DAVID
The results showed that DEGs in the blood of CP patients were involved in translation-related biological progress (BP) terms (Table 1a)

Summary

Introduction

This study aimed to identify characteristic representative genes through a comparative analysis of gene expression profiles in the blood and saliva of chronic periodontitis (CP) and refractory periodontitis (RP) patients to provide new treatment strategies that may be helpful in the treatment of different forms of periodontitis. Periodontal diseases have become a major health problem worldwide. Chronic periodontitis (CP) is an inflammatory destruction of tooth supporting structures that, if CP is found in the presence of subgingival calculus and local factors [11]. CP is initiated and maintained in the first line by complex poly-microbial infection, and the innate susceptibility of the patient is an accepted critical factor that will determine the destructive character of the disease [14]. With high incidence and prevalent characteristics, the diagnosis and treatment of CP and RP has undoubtedly been a challenge for both clinical investigators and clinicians

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