Abstract
Background. Detection of the pathogenicity factors is one of the actual directions in studying of the anthrax causative agent. First of all, it includes the presence of tripartite toxin, capsule and the related structural and regulatory genes. Their presence or absence is defined by structural changes in cellular structure of blood and immunocomplex organs of a macroorganism.Objective: To conduct a comparative analysis of subpopulational structure of marrow and peripheral blood cells at anthrax infection in experimental animals and to trace dynamics of the infectious process caused by Bacillus anthracis of different genotypes.Methods. Comparative examination of subpopulational structure of red marrow and peripheral blood cells of white mice in dynamics of the infectious process caused by B. anthracis of different genotypes was conducted. Also, neutrophil maturing index and leucoerythrocytic correlation were calculated in red marrow. Degree of manifestation of neutrophil pathological granularity was revealed in peripheral blood smears stained by the Freifeld’s method. Statistical processing of the received data was conducted using the computer program “Statistics”, version 6 (Novosibirsk). Authentic results were considered if the error probability was less than 0.05 (р < 0.05) in relation to the control.Results. It is revealed that alterations in white mice infected by B. anthracis strains with the same set of plasmids are significantly various.Conclusion. The resulted data permit to assume that the distinction of pathological alterations in peripheral blood and red marrow is probably connected with the other factors of the pathogen invasiveness like enzymatic activity, ability to adhesion and many others.
Highlights
Detection of the pathogenicity factors is one of the actual directions in studying of the anthrax causative agent
To conduct a comparative analysis of subpopulational structure of marrow and peripheral blood cells at anthrax infection in experimental animals and to trace dynamics of the infectious process caused by Bacillus anthracis of different genotypes
Comparative examination of subpopulational structure of red marrow and peripheral blood cells of white mice in dynamics of the infectious process caused by B. anthracis of different genotypes was conducted
Summary
В качестве объекта исследования использовали четыре штамма B. anthracis из музея живых культур ФКУЗ «Иркутский научно-исследовательский противочумный институт» Роспотребнадзора: B. anthracis И-275 (рХО1–/ рХО2–), B. anthracis И-323 (рХО1–/рХО2–), B. anthracis И-217 (рХО1+/рХО2–) и вакцинный аттенуированный штамм B. anthracis 34F2 Stern (рХО1+/рХО2–) [10]. Животные были разделены на четыре опытных группы (по 60 особей) и контрольную (20 особей). Белых мышей опытных групп инфицировали спорами культур B. anthracis однократно подкожно в область правого бедра в объёме 0,5 мл в дозе ЛД50. Контрольной группе животных в том же объёме вводили изотонический раствор хлорида натрия, рН 7,2. Показатель бактериемии оценивали посевом материала (кровь из сердца) на мясопептонном агаре с последующей идентификацией выделенных культур. Фиксированные мазки костного мозга окрашивали по Романовскому – Гимзе [11]. Мазки крови окрашивали по Романовскому – Гимзе и по методу Фрейфельда
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