Abstract
Orf is highly contagious zoonoses which is caused by the orf virus (ORFV). In order to determine the characteristics of B2L genes between vaccine strain and wild strain of ORFV in China. In this study we choose the weak vaccine of ORFV produced in biological products (Shandong Taifeng, China) and the wild strain (FJ-PN) isolated and identified in our laboratory to amplify, clone and sequence the B2L genes, and the changes in nucleotide, amino acid and secondary and tertiary protein structure of the B2L proteins were compared and analyzed by bioinformatics software. Comparison results showed that the nucleotide sequence similarity and amino acid sequence similarity of the B2L genes of vaccine strain and wild strain were 98.6% and 98.7% respectively. There was no significant difference between the primary, secondary and tertiary structures of the B2L proteins. The results of this study provide ideas for the study on the function and molecular biological characteristics of related proteins after the ORFV is weakened.
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