Abstract
DNA sequences related to avian leukosis virus (ev genes) were identified in the genome of chickens from 6 experimental strains including 3 randombred lines (Wyandotte, Fayoumi and White Leghorn), 2 divergently selected Rhode Island Red lines and a synthetic broiler line. Digestion of genomic DNA with Sad and BamHI enzymes and hybridization with either RAV-2 sequences or an LTR-gag probe revealed 66 different SacI bands, 53 of which could be associated with particular BamHI bands such that the ev loci could be defined at the molecular level. The distribution patterns were very strain-specific. Average band frequency and heterozygosity level could be correlated with the genetic history of the lines and their inbreeding level which varied from 0.04 to 0.28. Withinfamily segregation did not show any allelic relationship between retroviral insertions that were considered to be 2 genes. The Fayoumi line showed the largest variation in ev number per bird and contained a few birds free of any endogenous viral sequences, designated ev0. The broiler line showed the highest ev number. Very few ev loci were shared by different lines; only ev6 was found in all strains. Comparison with the established classification in White Leghorns was not straightforward and further analysis of DNA polymorphism and viral expression is needed.
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