Abstract
Aims: Retinoblastoma (Rb) is the most common intraocular malignancy of early childhood. Subpopulations of Rb cells express the stem cell marker ABCG2; yet little is known regarding the differences between ABCG2+ and ABCG2- subpopulations, particularly in threedimensional culture. The aim of this study was to compare ABCG2+ (stem-like) populations with ABCG2- cells in retinoblastoma in threedimensional, tumor-like populations. Methods: We compared aggregates of ABCG2+ and ABCG2- Rb cells to test the hypothesis that ABCG2+ cells would preferentially exhibit stem cell markers and less mature retinal marker expression as compared with ABCG2- cells. Retinoblastma cells (Y79 and WERI-Rb27) were immunomagnetically enriched into ABCG2+ and ABCG2- populations and grown as threedimensional aggregates. Enriched populations were examined as aggregates, along with embryoid bodies formed by iPSCs. Results: Immunostaining revealed that both ABCG2+ and
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