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Abstract
Genetic studies of plants are base on high efficiency of purified DNA samples. In this study, we optimized DNA extraction and PCR conditions of Satureja khuzistanica from Iran. The aerial organs of this plant contain high levels of essential oil which makes it difficult to DNA extraction with high quality and thereby intervened with subsequent PCR expansion. Four published DNA extraction protocols include Dellaporta (1983), Doyle and Doyle (1990), Murry and Thompson (1980), Kang and yang (2004) were compared for their capability to produce suitable quality DNA from Satureja khuzistanica . The protocol that provided the foremost DNA quality in the Satureja khuzistanica is selected. In Dellaporta method, no acceptable results were found because SDS buffer extraction was not attached to proteins well. In Doyle and Doyle, the obtained DNA was negligible. In kang and yang method, the quality of extracted DNA was not satisfying. Finally, in modified Murray and Thompson method, the extracted DNA has proper quality. In this method, these factors had the considerable effect on the quality of extracted DNA include: change of incubation time, high NaCl concentration, temperature changes in centrifugation stages, use of proteinase K and TE with high amount of salt and use of plant leaves before flowering stage.
Highlights
Different plant species often may not allow optimal DNA production from one extraction protocol
Different protocols for DNA extraction have been applied to many plant species which were modified to provide DNA extraction protocol, suitable for several kinds of genetic studies in plants (Doyle & Doyle, 1987; Wang & Taylor, 1993)
Medicinal and aromatic plants utilization and protection has attracted global attention. This plant contains secondary metabolites such as essential oils, flavonoids, alkaloids, phenols and terpenes which would interfere with the DNA extraction procedures (Abdollahi et al, 2003; Padmalatha & Prasad, 2006)
Summary
Different plant species often may not allow optimal DNA production from one extraction protocol. Satureja khuzistanica of the family Lamiaceae is an aborigine plant of Iran that is widely distributed in the West and South west Provinces of Iran (Jamzad, 1994) It is famous for its medical uses as painkiller and antiseptic in traditional medicine (Amanlou et al, 2004; Abdollahi et al, 2003; Hajhshemi et al, 2002). Medicinal and aromatic plants utilization and protection has attracted global attention This plant contains secondary metabolites such as essential oils, flavonoids, alkaloids, phenols and terpenes which would interfere with the DNA extraction procedures (Abdollahi et al, 2003; Padmalatha & Prasad, 2006). Due to medicinal and aromatic plants contain high amounts of many different secondary metabolites; it www.ccsenet.org/ijb
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