Comparative acetylome analysis reveals the potential roles of lysine acetylation for DON biosynthesis in Fusarium graminearum

Shanyue Zhou,Chunlan Wu

doi:10.1186/s12864-019-6227-7

Abstract

BackgroundFusarium graminearum is a destructive fungal pathogen of wheat, barley and other small grain cereals. During plant infection, the pathogen produces trichothecene mycotoxin deoxynivalenol (DON), which is harmful to human and livestock. FgGCN5 encodes a GCN5 acetyltransferase. The gene deletion mutant Fggcn5 failed to produce DON. We assumed that lysine acetylation might play a key regulatory role in DON biosynthesis in the fungus.ResultsIn this study, the acetylome comparison between Fggcn5 mutant and wild-type strain PH-1 was performed by using affinity enrichment and high resolution LC-MS/MS analysis. Totally, 1875 acetylated proteins were identified in Fggcn5 mutant and PH-1. Among them, 224 and 267 acetylated proteins were identified exclusively in Fggcn5 mutant and PH-1, respectively. Moreover, 95 differentially acetylated proteins were detected at a significantly different level in the gene deletion mutant:43 were up-regulated and 52 were down-regulated. GO enrichment and KEGG-pathways enrichment analyses revealed that acetylation plays a key role in metabolism process in F. graminearum.ConclusionsSeeing that the gens playing critical roles in DON biosynthesis either in Fggcn5 mutant or PH-1. Therefore, we can draw the conclusion that the regulatory roles of lysine acetylation in DON biosynthesis in F. graminearum results from the positive and negative regulation of the related genes. The study would be a foundation to insight into the regulatory mechanism of lysine acetylation on DON biosynthesis.

Highlights

Fusarium graminearum is a destructive fungal pathogen of wheat, barley and other small grain cereals
We can draw the conclusion that the regulatory roles of lysine acetylation in DON biosynthesis in F. graminearum results from the positive and negative regulation of the related genes
To gain insights into the possible acetylome regulated by FgGCN5 in F. graminearum, we generated the gene deletion construct by the splitmarker approach [24] and transformed it into the wildtype strain PH-1

Summary

Introduction

Fusarium graminearum is a destructive fungal pathogen of wheat, barley and other small grain cereals. The pathogen produces trichothecene mycotoxin deoxynivalenol (DON), which is harmful to human and livestock. Fusarium graminearum is a disastrous fungal pathogen which causes Fusarium head blight (FHB) on wheat, barley and other small grain cereals [1, 2]. In addition to the severe yield loss and quality damage, the pathogen produces trichothecene-type mycotoxins, such as deoxynivalenol (DON) in the infected tissue. Most enzymes involved in glycolysis, the tricarboxylic (TCA) cycle, gluconeogenesis, the urea cycle, and fatty acid metabolism were acetylated in human liver tissue [22]. The protein acetylation is involved in the secondary metabolism process, such as nonribosomal peptide synthesis, hydroxamate siderophore and phosphinic acid products biosynthesis [20]

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