COMPARAÇÃO ENTRE AS TÉCNICAS ANATÔMICAS DE FORMOLIZAÇÃO, GLICERINAÇÃO E PREENCHIMENTO/CORROSÃO NO SISTEMA RESPIRATÓRIO DE CÃES

Abstract

The objective of the techniques of conservation of anatomical pieces is to preserve the morphology, consistency, color and flexibility of the same. Among the techniques currently used are formaldehyde, glycerin and fill/corrosion, which were compared in the present study. Four pieces were used in the evaluation of each technique. For formalinization, the lungs were washed, dissected and fixed in 10% formalin. For the glycerination technique, the organs were fixed in 10% formaldehyde, washed for 48 hours, and then dried in the shade. Sequentially, the organs were submerged in hydrogen peroxide 10 and again dried in the shade. Then, they remained submerged in absolute alcohol for two months, were removed, dried naturally, and submerged in glycerin for 30 days. For the filling/corrosion technique the lungs were washed, then submerged in 125 Colloquium Agrariae, vol. 13, n. Especial 2, Jan–Jun, 2017, p. 124-128. ISSN: 1809-8215. DOI: 10.5747/ca.2017.v13.nesp2.000217 alcohol for 24 hours. The trachea was cannulated for the fixation step, through the canula a continuous air stream containing alcohol and formalin mixture was introduced into the bronchial tree. Subsequently, the acrylic was inserted, the pieces were placed in the water for 24 hours, and soon the corrosion was carried out with hydrochloric acid. The glycerolation technique proved to be more advantageous when compared to formaldehyde, since it presents a low toxicity and irritation index, which facilitated the management and study, besides requiring a low degree of maintenance. However, for the visualization of the bronchial tree, the filling/corrosion technique proved to be superior to the others, but this technique demands greater care during its handling and storage.