Comparação entre as Respostas do Clone 4430 e Tradescantia pallida no Teste de Micronúcleos (Trad-MN)

Abstract

Comparison of responses of the Clone 4430 and Tradescantia pallida in the Micronucleus Assay (Trad-MN) The Genotoxicity Assay with clone 4430 genus Tradescantia is utilized worldwide in the Tradescantia Micronucleus assay (Trad-MN). In Brazil, several Trad-MN studies have utilized Tradescantia pallida to replace the clone 4430. However, in the literature, only one comparison between the two species was performed, and more studies are necessary to examine the mutagenic potential of Tradescantia pallida for application in the Trad – MN. The present work aims to compare the results of Trad-MN assays between the two plants, in the testing of mutagenic substances (positive controls) and water (as negative controls), to evaluate the utilization potential of Tradescantia pallida for replacing clone 4430. The results obtained showed that Tradescantia pallida can replace clone 4430 in the micronucleus assay, however, the substances testing showed high variation in the micronuclei induction (3% – 8% for negative control and 5% – 11% for positive control), indicating low discriminating potential of the assay and limited applicability in monitoring programs.