Abstract
Three selective enrichment broths - selenite cystine (SC), Muller-Kauffmann tetrathionate (MKT) and Rappaport-Vassiliadis (RV) - were compared, for Salmonella Typhimurium isolation from rectal swabs of a calf experimentally infected. The bacteriological procedure involved pre-enrichment in Hajna-GN broth (only for the samples inoculated in RV broth), selective enrichment (SC, MKT and RV broths), culture in modified brilliant green agar (BGA), presumptive biochemistry tests (using triple-sugar-iron agar and lysine-agar) and slide agglutination test with poli-O and poli-H Salmonella antisera. SC and MKT broths were more efficient in the isolation of Salmonella Typhimurium (12 positive samples), whereas RV broth had a lower efficiency in the microbiological isolation (ten positive samples).
Highlights
The bacteriological procedure involved pre-enrichment in Hajna-GN broth, selective enrichment (SC, Muller-Kauffmann tetrathionate (MKT) and RV broths), culture in modified brilliant green agar (BGA), presumptive biochemistry tests and slide agglutination test with poli-O and poli-H Salmonella antisera
selenite cystine (SC) and MKT broths were more efficient in the isolation of Salmonella Typhimurium (12 positive samples), whereas RV broth had a lower efficiency in the microbiological isolation
selenite cystine (SC) and Muller-Kauffmann tetrathionate (MKT) broths were more efficient in the isolation of Salmonella Typhimurium (12 positive samples), whereas RV broth had a lower efficiency in the microbiological isolation (ten positive samples)
Summary
Three selective enrichment broths selenite cystine (SC), Muller-Kauffmann tetrathionate (MKT) and Rappaport-Vassiliadis (RV) were compared, for Salmonella Typhimurium isolation from rectal swabs of a calf experimentally infected.
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