Abstract
The increase in animal production has increased the amount of effluents destined, most of times, to agriculture and aquatic resources. As the same of others enteric microorganisms, Salmonella is present in feces. In the environment Salmonella sp. become an important source of transmission and, consequently, a risk to animal production and public health. In this experiment, three methodologies for quantifying Salmonella sp. were compared, so as to determine the best estimation technique of the Most Probable Number (MPN) to be applied in liquid waste from effluent treatment systems. In this study, were used five water samples from an aquaculture tank. Compared to the bacterial count of the Salmonella Typhimurium’s inoculum (10 5 a 10 6 CFU), it was verified that the average MPN (4.441 CFU) in method A is very similar to those counts (P>0.05). On the other hand, the average MNP observed in method B (1.380 CFU) and C (3.204 CFU) are statistically different from them (P<0.001 and P<0.01, respectively). Considering that method A was the one to present MPN values closest to the inoculated amounts, the employment of this methodology is suggested for the analysis of effluents.
Highlights
The increase in animal production has increased the amount of effluents destined, most of times, to agriculture and aquatic resources
As the same of others enteric microorganisms, Salmonella is present in feces
Compared to the bacterial count of the Salmonella Typhimurium’s inoculum (105 a 106 CFU), it was verified that the average Most Probable Number (MPN) (4.441 CFU) in method A is very similar to those counts (P>0.05)
Summary
Utilizaram-se cinco amostras do efluente do Tanque para manutenção de estoque de peixes do Setor de Aquicultura da Universidade, onde foi determinada ausência de salmonelas [19]. 0,1mL, 1mL e 10mL do efluente foram inoculados em 10mL, 99mL e 90mL de Água Peptonada Tamponada; estas foram incubadas a 37oC/24h; de cada diluição 0,1mL foi transferido para 9,9 mL de caldo RV e incubados a 42oC/18; de cada. De cada tubo de RV, com auxílio de uma alça de platina, uma alíquota foi semeada em placa de Ágar XLD, as quais foram incubadas a 37oC/24h. De cada placa de XLD, pelo menos uma colônia com características morfológicas de salmonela foi repicada para confirmação de gênero [19]. As placas de XLD com confirmação de crescimento de Salmonella foram relacionadas às respectivas diluições em caldo Rappaport Vassiliadis (RV) ou Água Peptonada e organizadas segundo o número de tubos positivos em cada diluição, para determinação do NMP correspondente [8,17,29]. Número de unidades formadoras de colônias (Log10) de Salmonella Typhimurium inoculadas e recuperadas (NMP) em meio líquido, segundo o método utilizado
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