Abstract
This study aimed to evaluate different methods for culturing sputum, Ogawa-Kudoh (O - K) and Petroff, using reduced time for decontamination (Petroff - R), expecting to know their effectiveness in tuberculosis laboratory diagnosis. Four hundred and fifty-nine sputum samples of patients with pulmonary symptoms were cultured using O - K and Petroff - R, to be compared with the regular Petroff method. O - K and Petroff - R methods showed efficient sensitivity in isolating Mycobacterium tuberculosis and micobacteria other than tuberculosis, even in samples with negative acid fast smear. Statistics analysis showed similar contamination rate for O - K when compared to Petroff. These data suggest that O-K, being easy to perform, having low costs, presenting low risk of worker contamination and good sensitivity in detecting M. tuberculosis, should be used in clinical laboratories as a good resource at tuberculosis diagnosis
Highlights
A tuberculose é uma doença infecto contagiosa conhecida há séculos e continua sendo um importante problema de saúde pública (Ministério da Saúde, 1992; Snider et al, 1994)
This study aimed to evaluate different methods for culturing sputum, Ogawa-Kudoh (O-K) and Petroff, using reduced time for decontamination (Petroff-R), expecting to know their effectiveness in tuberculosis laboratory diagnosis
Statistics analysis showed similar contamination rate for O-K when compared to Petroff
Summary
As amostras de escarros foram obtidas de pacientes sintomáticos respiratórios com suspeita clínica de tuberculose, atendidos nas Unidades Básicas de Saúde do município de Maringá/Paraná e cidades vizinhas e encaminhadas ao Laboratório de Ensino e Pesquisa em Análises Clínicas da Universidade Estadual de Maringá (Lepac), Estado do Paraná, no período de junho de 2004 a março de 2005. Foram analisadas 459 amostras de escarro, de 298 pacientes sintomáticos respiratórios. As amostras foram coletadas de acordo com as normas recomendadas pelo Laboratório Nacional de Referência Prof. Hélio Fraga, Rio de Janeiro, Estado do Rio de Janeiro (Ministério da Saúde, 1994), conservadas a temperatura de 4 – 10oC e processadas até um Cultivo para diagnóstico da tuberculose período máximo de cinco dias
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