Abstract
AbstractComparison of phosphatase activities in ectomycorrhizal Homobasidiomycetes cultured in vitro. Phosphatase activities, measured on intact mycelia (accessible activities) or on mycelial homogenates (total activities), were compared in twelve ectomy corrhizal strains of Homobasidiomycetes (Hebeloma cylindrosporum, Laccaria laccata; Suillus bellini; S. bovinus; S. granulatus; S. luteus, Pisolithus arhizus). The effects of the following factors were examined: (i) two orthophosphate concentrations in the culture medium; (ii) two incubation substrates (sodium p‐nitrophenylphosphate or sodium phytate). Phosphatase activities were highest when mycelial homogenates were incubated in the presence of p‐nitrophenyl phosphate after culturing the mycelia on medium slightly deficient in orthophosphate, particularly in the case of one S. granulatus isolate.
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