Abstract
Reports in the literature (3, 12, 13) suggest that it is feasible to replace with an ELISA test, the more cumbersome and expensive method of in-vivo neutralization for the assessment of the potency of antivenoms. In this paper we compare both methods for the antivenom Crotalus durissus terrificus. We used purified Gamma-globulins and complete sera, and with neither was found any correlation between the methods tested. A plausible explanation is that while the in-vivo neutralization test is measuring antitoxin antibodies the ELISA test is measuring total antibodies. We suggest to verify the correlation using pure toxin as the antigen for ELISA. We do not recommend at this stage, contrary to other authors suggestions (3), the use of ELISA for assessment of titers of antivenom Crotalus durissus terrificus.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.