Comparable Results between Continuous and Sequential Media on Mouse Embryo Cultured in Time-Lapse Incubator

Abstract

Time lapse incubator now became a new technology for clinical In Vitro Fertilization. This incubator allows embryo observation continuously and periodically without taking the embryo out from the incubator. The nature function of time lapse incubator requires continuous incubation without taking out the embryo from the incubator, means there is no change over media during incubation. In the other hand most culture media that available in the market distinguish between cleavage and blastocyst stage embryo. This known as sequential media. This experiment compared the use of continuous and sequential media during in vitro embryo culture using time lapse incubator. One cell mouse embryo derived from F1 (C57BL/J MARP x CBA/MARP) were used in this experiment. Embryos were culture for 5 days until they reach blastocyst stage. The continuous media (Global Media, Life Global) was used to culture media from day 1 till day 5, while sequential media were divided into two parts. Cleavage media (SIVF-Cleavage, Cook Medical, Brisbane) was used from day 1 till day 3, and Blastocyst media (SIVF-Blastocyst, Cook Medical, Brisbane) was used from day 3 till day 5. Control embryos were cultured in sequential media (SIVF- Cleavage and SIVF-Blastocyst, Cook Medical) in bench top incubator (MINC, Cook, Brisbane). A total of 320 one cell embryos were used in this experiment. Embryo development was evaluated by the number of embryo developed into blastocyst.