Abstract
Summary We study the self-assembly dynamics of human progenitor cells in agarose microwells that are used for production of chondrogenic organoids. Using image analysis on time-lapse microscopy, we estimate the aggregate area in function of time for a large number of aggregates. In control conditions, the aggregate radius follows an exponential relaxation that is consistent with the dewetting dynamics of a liquid film. Upon introducing Y-27632 Rho kinase inhibitor, the compatibility with the liquid model is lost, and slowed-down relaxation dynamics are observed. We demonstrate that these aggregates behave as granular piles undergoing compaction, with density relaxation that follows a stretched exponential. Using simulations with an individual cell-based model, we construct a phase diagram of cell aggregates that suggests that the aggregate in presence of Rho kinase inhibitor approaches glass transition.
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