Compact surface plasmon resonance imaging sensing system based on general optoelectronic components

Abstract

We present a simple surface plasmon resonance imaging (SPRi) sensing system based on some common optoelectronic devices in this paper. Using an optical fiber based SPR sensor as sensing element in our system, the SPRi system is dramatically compact. A small universal LED is used as the light source. The light intensity is record as images that can be captured by a simple web camera. A Microsoft Visual C++6.0 based Windows software program is written to process the image data which contain SPRi information. Experimental results show that the relationship between the relative intensity and RI is a linear relation in a RI range from 1.3396 to 1.3645. Using this SPRi device, we measure the specific binding between the Con A and RNase B, which demonstrates its capability for biomedical selective affinity monitoring. The proposed SPRi sensing system also has the capacity for biochemical multiple channel measurement with further investigation.