Compact lensless subpixel resolution large field of view microscope.

Abstract

We report on a method to increase the spatial resolution in a compact lensless microscope. A compact side illumination is fabricated to illuminate the sample with a collimated beam by diffraction from a volume phase grating. The wavelength of a semi-conductor laser source (vertical-cavity surface-emitting laser) is tuned with the injection current to alter the illumination direction by wavelength selective diffraction from the volume phase grating. The angle tuning is such that several subpixel shifted digital inline holograms are obtained. The stack of holograms is then processed in a pixel super-resolution reconstruction algorithm. The amplitude of the sample is reconstructed with subpixel resolution over a large field of view (FOV). The technique is demonstrated on a 1951 USAF test target. A resolution of ∼2.76 μm, over a FOV of ∼28 mm2, is demonstrated for a device of <2 cm height. The original pixel size was 5.2 μm demonstrating the subpixel resolution.