Abstract
Square wave pulses have been identified as more lethal compared to exponential decay pulses in PEF applications. This is because of the on-time which is longer causes a formidable impact on the microorganisms in the food media. To have a reliable high-voltage pulse generator, a technique of capacitor discharge was employed. Four units of capacitor rated 100 μF 1.2 kV were connected in series to produce 25 μF 4.8 kV which were used to store the energy of approximately 200 J. The energy stored was discharged via HTS 181-01-C to the load in the range of nano to microseconds of pulse duration. The maximum voltage applied was limited to 4 kV because it is a lab-scale project. The electrical circuit diagram and the development procedure, as well as experimental results, are presented.
Highlights
Cell electroporation therapy (EPT), genetic therapy, ultrasonic cleaning, chemical-free bacterial decontamination, and medical imaging are the examples of vast applications that require a high-voltage pulse generator which can provide pulse width in a scale of a nanosecond, microsecond, and millisecond [1,2,3,4,5,6,7,8,9,10,11]
The cell loses its homeostasis and eventually dies if and only if the strength of the electric field applied is sufficiently intense [19]. This method was used as a tool for microbial inactivation [20, 21]. e impact of the pulsed electric field (PEF) on microbial viability has been widely studied in various types of bacteria including Gram-positive and -negative bacteria, yeast, protozoan parasites, and spores [22,23,24,25,26,27,28,29]
The properties of the generated pulse can be assessed from the perspective of the pulse amplitude, pulse width, and rise and fall time. is is crucial to be accomplished because, subsequently, it will be a benchmark for practical testing
Summary
Cell electroporation therapy (EPT), genetic therapy, ultrasonic cleaning, chemical-free bacterial decontamination, and medical imaging are the examples of vast applications that require a high-voltage pulse generator which can provide pulse width in a scale of a nanosecond, microsecond, and millisecond [1,2,3,4,5,6,7,8,9,10,11]. A commercial electroporator is capable of providing high electric field intensity in the order of 10 kV/cm–100 kV/cm with continuous operation and high repetition rate, it does not fit in the studies on bacterial inactivation or various substances extraction from cells [45]. For this reason, the aim of this study was to develop a square-wave high-voltage pulse generator that. It suppresses the overshoot of the output pulse voltage across the load during turn-on time while escalating the minimum load that can be handled by the generator. e treatment chamber is made equivalent to the RC circuit connected in parallel which represents charge carrying conduction and dielectric polarization, respectively
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