Dr. Cline was looking for a way to revitalize the class project in her environmental microbiology course, an upper-division college class that introduces students to general microbiology in the first 5 weeks and then explores environmental applications of microbiology. Dr. Elliott was looking for volunteers to participate in a community stream monitoring project. Cline was intrigued by service learning as a way to engage students and widen their focus to the world outside the classroom, but hesitant to sacrifice the time they could otherwise devote to studying fundamental concepts. And furthermore, could students who had only studied microbes for weeks give back to their community in a meaningful way? The answer proved to be yes – as long as the project was carefully designed.Phytophthora ramorum Werres, De Cock & Man in’t Veld 2001 is an invasive pathogenic oomycete that attacks a wide range of plants (Figure 1; Cline et al., 2008). Since its introduction in the 1990s, it has become established in California and Oregon and has been detected in nurseries in Washington (Rizzo et al., 2002). Because its flagellated spores disperse by swimming, stream leaf baiting is an effective way to detect the pathogen within a watershed. Researchers from Washington State University Puyallup, including Dr. Elliott, have implemented a stream baiting project in which community members deploy Rhododendron leaves in mesh bags for 1 week and then culture Phytophthora spp. from leaf disks on selective media and screen for P. ramorum. This allows for early detection of P. ramorum and other invasive Phytophthora spp., as well as examination of the biodiversity of Phytophthora spp. in stream ecosystems.For the class project, we completed the standard leaf baiting in First Creek, an urban stream, and Puget Creek, an urban park, both in Tacoma, Washington. Each group was responsible for one leaf from each creek. After the baiting, culturing, and identification of Phytophthora spp. was completed by the students, with the assistance of Elliott, DNA sequencing was used to generate a phylogenetic tree of Phytophthora. A detailed student lab handout and instructor manual are available at https://sites.google.com/a/uw.edu/erica-cline-uwt-faculty-webpage/home.While we were relieved to not find P. ramorum in our local streams, we did find several other Phytophthora spp., including both saprophytes and pathogens. The students presented their results to a group of middle school students from First Creek who are leading a restoration effort for the creek.Subsequently, students tested salt tolerance of P. ramorum, finding that a common lineage produces more chlamydospores when cultured on salt medium. This finding has important management implications, as the pathogen may be able to disperse in salt water, demanding increased vigilance in enforcing the quarantine (see detailed project description at http://www.puyallup.wsu.edu/ppo/sod/monitoring/student_projects/index.htm).Tips for successful implementation of service learning projects:

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call