Community respiration/production and bacterial activity in the upper water column of the central Arctic Ocean

Abstract

Community metabolism (respiration and production) and bacterial activity were assessed in the upper water column of the central Arctic Ocean during the SHEBA/JOIS ice camp experiment, October 1997–September 1998. In the upper 50 m, decrease in integrated dissolved oxygen (DO) stocks over a period of 124 d in mid-winter suggested a respiration rate of ∼3.3 nM O 2 h −1 and a carbon demand of ∼4.5 gC m −2. Increase in 0–50 m integrated stocks of DO during summer implied a net community production of ∼20 gC m −2. Community respiration rates were directly measured via rate of decrease in DO in whole seawater during 72-h dark incubation experiments. Incubation-based respiration rates were on average 3-fold lower during winter (11.0±10.6 nM O 2 h −1) compared to summer (35.3±24.8 nM O 2 h −1). Bacterial heterotrophic activity responded strongly, without noticeable lag, to phytoplankton growth. Rate of leucine incorporation by bacteria (a proxy for protein synthesis and cell growth) increased ∼10-fold, and the cell-specific rate of leucine incorporation ∼5-fold, from winter to summer. Rates of production of bacterial biomass in the upper 50 m were, however, low compared to other oceanic regions, averaging 0.52±0.47 ngC l −1 h −1 during winter and 5.1±3.1 ngC l −1 h −1 during summer. Total carbon demand based on respiration experiments averaged 2.4±2.3 mgC m −3 d −1 in winter and 7.8±5.5 mgC m −3 d −1 in summer. Estimated bacterial carbon demand based on bacterial productivity and an assumed 10% gross growth efficiency was much lower, averaging about 0.12±0.12 mgC m −3 d −1 in winter and 1.3±0.7 mgC m −3 d −1 in summer. Our estimates of bacterial activity during summer were an order of magnitude less than rates reported from a summer 1994 study in the central Arctic Ocean, implying significant inter-annual variability of microbial processes in this region.