Abstract
AbstractA study has been completed examining design issues concerning the interpretation of and dissemination of multimodal medical imaging data sets to diverse audiences. To create a model data set mouse fibrosarcoma tissue was visualised via magnetic resonance imaging (MRI), Matrix-Assisted Laser Desorption/Ionisation-Mass Spectrometry (MALDI-MSI) and histology. MRI images were acquired using the 0.25T Esaote GScan; MALDI images were acquired using a Q-Star Pulsar I mass spectrometer. Histological staining of the same tissue sections used for MALDI-MSI was then carried out. Areas assigned to hemosiderin deposits due to haemorrhaging could be visualised via MRI. In the MALDI-MSI data obtained the distribution sphingomyelin species could be used to identify regions of viable tumour. Mathematical ‘up sampling’ using hierarchical clustering-based segmentation provided a sophisticated image enhancement tool for both MRI and MALDI-MS and assisted in the correlation of images.
Highlights
Images of the human body in vivo are routinely produced to reveal, diagnose, or examine disease
We have previously reported observation of the tumour haemorrhaging induced by this compound by matrix-assisted laser desorption ionisation-mass spectrometry imaging (MALDIMSI) and conventional histology [12]
Chemicals and materials a-Cyano-4-hydroxycinnamic acid (CHCA), aniline (ANI), ethanol (EtOH), chloroform (CHCl3), acetonitrile (ACN), octyl-a/b-glucoside (OcGlc), tri-fluoroacetic acid (TFA), ammonium bicarbonate, haematoxylin, eosin, xylene and DPX mountant were from Sigma-Aldrich (Dorset, UK)
Summary
Images of the human body (or its parts) in vivo are routinely produced to reveal, diagnose, or examine disease. Ex vivo imaging of excised organs and tissues is routinely performed for medical purposes, as a part of pathological screening. A large number of sophisticated in vivo imaging modalities exist including radiography, MRI and ultrasound, and for removed tissues the standard procedure employed is histological staining, increasingly supported by mass spectrometry [1,2,3,4,5,6]. Matrix-Assisted Laser Desorption/Ionisation-Mass Spectrometry (MALDI-MSI) is a powerful technique allowing visualisation of the spatial distribution of a particular species, within a biological tissue sample. MALDI-MSI has been frequently utilised for the direct protein profiling of tumour tissue samples, including tumour margin analysis [7,8,9,10]
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