Common time–frequency analysis of local field potential and pyramidal cell activity in seizure-like events of the rat hippocampus

Abstract

To study cell-field dynamics, physiologists simultaneously record local field potentials and the activity of individual cells from animals performing cognitive tasks, during various brain states or under pathological conditions. However, apart from spike shape and spike timing analyses, few studies have focused on elucidating the common time–frequency structure of local field activity relative to surrounding cells across different periods of phenomena. We have used two algorithms, multi-window time frequency analysis and wavelet phase coherence (WPC), to study common intracellular–extracellular (I–E) spectral features in spontaneous seizure-like events (SLEs) from rat hippocampal slices in a low magnesium epilepsy model. Both algorithms were applied to ‘pairs’ of simultaneously observed I–E signals from slices in the CA1 hippocampal region. Analyses were performed over a frequency range of 1–100 Hz. I–E spectral commonality varied in frequency and time. Higher commonality was observed from 1 to 15 Hz, and lower commonality was observed in the 15–100 Hz frequency range. WPC was lower in the non-SLE region compared to SLE activity; however, there was no statistical difference in the 30–45 Hz band between SLE and non-SLE modes. This work provides evidence of strong commonality in various frequency bands of I–E SLEs in the rat hippocampus, not only during SLEs but also immediately before and after.