Abstract
A rat genomic DNA segment containing the angiotensinogen gene has been isolated from a gene library by hybridization with a restriction fragment derived from a previously cloned cDNA for rat angiotensinogen. Restriction mapping and nucleotide sequence analysis of the cloned DNA fragments have indicated that the rat angiotensinogen gene is approximately 11.8 kilobase pairs long and consists of five exons separated by four introns. Because it has recently been reported that the sequence of angiotensinogen significantly resembles those of alpha 1-antitrypsin and ovalbumin and also that the exon-intron arrangements of the alpha 1-antitrypsin and ovalbumin genes are completely different, this study compares the structural organization of the angiotensinogen gene with those of the alpha 1-antitrypsin gene and the ovalbumin gene. The comparison has revealed that the four introns of the angiotensinogen gene are all located at the positions equivalent to or corresponding to those observed in the alpha 1-antitrypsin gene, suggesting that the angiotensinogen gene and the alpha 1-antitrypsin gene have diverged from a common ancestor gene. This finding raises interesting possibilities regarding the biological function of angiotensinogen and the evolution of the angiotensinogen gene.
Highlights
Faculty of Medicine, Kyoto606, Japan share an identical structural organization in the number and positions of introns
The hybridization probe used was reported that the sequence of angiotensinogensignifi- the AccI fragment of 1097 base pairs containing nucleotide residues cantly resembles thoseof al-antitrypsin and ovalbumin 81-1,177 of rat angiotensinogen cDNA, whicwh as excised from clone aafennrdteitnartly,spothstiihsnasattuntdhdyeovecaxolombnup-mianritnersogntehanererssatanrrgueecmtcuoermnatlpslooerftgetalhyneidzaia1ft--ionppp(1llRaa0qsa)mug. eHli6dpy(pub2Brr)iifdRaiic3nza2adt2tiiowownnaas-(slp1apo1bese)ri.lfteSoidvruwmebpicethlhdoagnaeisnc[dgluoeos-nfc~ersg’ibewPnee]odr~mebCyiiscoTDMblyaPNateAnndiicaktfirbsatyergatmrneasepl.lnea(att1stie1oidn)n. of the angiotensinogen gene with those of the al-anti- All of the cloning procedures were conducted in accordance withthe trypsin gene and the ovalbumingene
Gen gene are all located at the positions equivalent to Restriction Mapping, Blot Hybridization, and Nucleotide Sequence or corresponding to those observed in thael-antitryp- Analysis-Restrictionendonucleases were purchasedfrom Takara sin gene, suggesting that the angiotensinogen gene andShuzo Co., Bethesda Research Laboratories, andNew England Biothe al-antitrypsin gene have diverged from a common ancestor gene
Summary
From the Institute for Immunology, Kyoto University that the angiotensinogengene and the al-antitrypsin gene. Faculty of Medicine, Kyoto606, Japan share an identical structural organization in the number and positions of introns. A rat genomic DNA segment containing the angiotensinogen gene has been isolated froma gene library by hybridization with a restriction fragment derived from a previously cloned cDNA for rat angiotensinogen. Restrictionmappingandnucleotidesequence analysis of the cloned DNA fragments have indicated thattheratangiotensinogen gene is approximately 11.8 kilobase pairs longand consists of five exons
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